Correlation between cytosolic free calcium, contracture, ATP, and irreversible ischemic injury in perfused rat heart.
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چکیده
منابع مشابه
Relationship between adenine nucleotide metabolism and irreversible ischemic tissue damage in isolated perfused rat heart.
The relationship between energy metabolism and the extent of irreversible ischemic damage was examined in an isolated perfused working rat heart. The amount of cardiac work recovered after reperfusion of hearts exposed to severe global ischemia was dependent upon both the duration of ischemia and the type of substrate provided (either 5 mM glucose or 5 mM glucose + acetate). There appear to be ...
متن کاملElevation in cytosolic free calcium concentration early in myocardial ischemia in perfused rat heart.
Changes in cytosolic free calcium concentration during myocardial ischemia were measured by 19F NMR in 5FBAPTA-loaded perfused rat hearts. The hearts were perfused with Krebs-Henseleit buffer containing 5 microM of the acetoxymethyl ester of 5FBAPTA, which was hydrolyzed by cytosolic esterases to achieve cytosolic concentrations of 5FBAPTA of 0.12 to 0.65 mM. Cytosolic free calcium concentratio...
متن کاملInjury in Perfused Rat Heart
The relations between ATP depletion, increased cytosolic free calcium concentration ([Ca;]), contracture development, and lethal myocardial ischemic injury, as evaluated by enzyme release, were examined using 19F nuclear magnetic resonance to measure [CaiJ in 1,2-bis(2amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid (5F-BAPTA)-loaded perfused rat hearts. Total ischemia at 370 C was induc...
متن کاملContracture and the calcium paradox in the rat heart.
The role of contracture in the manifestation of calcium paradox-induced damage was examined using 2,3-butanedione monoxime (BDM) to inhibit myofibrillar activity. Calcium and sodium gain, loss of intracellular components, and changes in structure were monitored. If 30 mM BDM was added at the time of calcium repletion after 10 minutes of calcium-free perfusion, some protection was afforded, part...
متن کاملRelationship between calcium circulation-related factors and muscle strength in rat sciatic nerve injury model
Objective(s): The purpose of this study is to investigate the indication function of the calcium circulation-related factors on the damage to muscle strength and contraction function after nerve injury. The target factors include ryanodine receptor (RyR), inositol-1,4,5-triphosphate receptor (IP3R), phospholamban (PLN), cryptocalcitonin (CASQ), ATPase and troponin C (T...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 1990
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.66.1.135